In the most advanced life scientific research, observing the molecular or protein behavior in live creatures’ cells becomes more and more important to analyze the mechanism of cancer or searching for the candidates of pharmaceutical drug. One of the observing methods is fluorescent in vivo imaging with a cooled CCD camera system. LAS-4000 is recommended for acquiring in vivo images of live small animals. To the details This is the new field emerged in the post-genome era. The proteomics includes the research area of proteins expressed from genes or proteins in general. The 2D electrophoresis followed by image analysis and further qualitatively analyzed by mass spectrometry is the general strategy towards proteomics accepted by many researchers now. To the details A new method for the detection of thousands of RNAs at one time. Several thousands to ten thousands of different cDNAs are fixed on a small area such as a glass slide. On the other hand, RNAs in the sample are processed for reverse transcription and fluorescently labeled. After hybridization of the fluorescently labeled cDNA and the microarray slide, image analysis of the glass slide is done by a high resolution fluorescence detector. To the details A very widely known method for the detection of a specific sequence in DNA. This method was named after Dr.E.M.Southern who introduced this method in 1975. After the gel electrophoresis of the sample DNA, blotting to a membrane is done and DNAs are transcribed to the membrane. A DNA probe of specific sequence is hybridized with the membrane. When the probe is a radioisotope (RI), exposure to phosphor imaging plate (IP) and detection by BAS system is done. When the probe is digoxigenine (DIG), a sandwich method using enzyme (ALP or HRP) tagged anti-DIG-antibody and chemilminescent detection by using chemiluminescence substrate is done. High sensitive detection of chemiluminescence is effectively done by a cooled CCD camera system. To the details To the details To the details To the details A widely used method for the detectionof specificprotein after electrophoresis. The sample is electrophoresed on a polyacrylamide gel, then, blotted to a membrane. The membrane is incubated with the antibody to the specific protein. Further , the secondary antibody labeled with an enzyme such as HRP (or ALP) is added and reacted. Chemiluminescence detection of the specific protein can be done by applying chemiluminescent substrate of the enzyme. To the details To the details To the details To the details A widely used detection method for a specific RNA after electrophoresis. The electrophoresis gel is blotted to a membrane and the transcribed RNA is detected by hybridization with a labeled cDNA probe. Phosphor imaging plate (IP) is used for the detection of RI probes, and very high sensitive cooled CCD camera system is used for detection of chemiluminescent probes. The amount of RNA in a cell is much less than DNA, and RNA is easily degraded by RNAse existing elsewhere. So, the handling of RNA must be done under extreme care. Because we can get molecular weight information from the electrophoresis length, Northern blotting is useful for verification purposes. This is the merit of Northern blotting compared to other methods such as microarray method. To the details To the details To the details To the details A new tool inevitable for visualizing various processes of metabolism in a cell. The origin of GFP was from a jelly fish (Aequorea victoria). A green fluorescence appeared after excitation of blue light. Various variants are available now. Fluorescent scanner or CCD camera system with appropriate excitation light source can be used for the imaging of GFP. To the details To the details To the details To the details BACK