Whole body autoradiography to analyze metabolism of 14C labeled compound Nuclides: 14C IP: BAS-MS2040 Excitation wavelength: 650nm Filter: B390 PMT-1 Sensitivity: S10000 Pixel size: 50μm Data: courtesy of Institute of Whole Body Metabolism Detection of modified bases in RNAs by primer extension method Primer extension analysis of E. coli 23S rRNA which was treated with or without CMC(N-cyclohexyl-N ' -β-(4-methylmorpholinium)ethycarbodiimide p-tosylate) to detect pseudouridine (Ψ) (left panel). As a control (right panel), the same analysis was performed against E. coli strain in which rluD (RNA-pseudouridylase) was depleted. m3Ψ stands for 3-methyl pseudouridine. Sample: ribosome RNA Nuclides: 32P IP: BAS-MS2040 Exposing time: one night Excitation wavelength: 650nm Filter: B390 PMT-1 Sensitivity: S10000 Pixel size: 50μm Data:  courtesy of Graduate School of Engineering, the University of Tokyo Tsutomu Suzuki, Ph.D., Naomi Hirabayashi SYBR® Green stained agarose gel electrophoresis of DNA Sample: /HindIII Excitation wavelength: 473nm Filter: Y520 PMT-1, 500V Pixel size: 50μm SYPRO® Ruby stained 2D gel electrophoresis of Saccharomyces cerevisiae 1st dimension:  Low pH isoelectric electrophoresis kit (Nacalai Tesque, Inc.) 2nd dimension:  17% low BIS SDS-PAGE Sample: Saccharomyces cerevisiae Excitation wavelength: 473nm Filter:O580 PMT-2, 500V Pixel size: 50μm Data:  courtesy of Yoshihiro Yamamoto, Industrial Technology Center, Kyoto Municipal Industrial Research Institute PAGE of Cy™3 labeled primer Sample: Cy™3 labeled primer Density:  500fmol / 200fmol / 100fmol / 50fmol / 20fmol / 10fmol / 5fmol / 2fmol / 1fmol Excitation wavelength: 532nm Filter: O580 PMT-1, 800V Pixel size: 50μm PAGE of Cy™5 labeled primer Sample: Cy™5 labeled primer Density:  500fmol / 200fmol / 100fmol / 50fmol / 20fmol / 10fmol / 5fmol / 2fmol / 1fmol Excitation wavelength: 635nm Filter: R670 PMT-1, 800V Pixel size: 50μm Silver-stained protein by SDS-PAGE Sample: BSA Density:  molecular-weight marker / 3μg / 1μg / 300ng / 100ng / 30ng / 10ng / 3ng / 1ng Excitation wavelength: 473nm Filter: Y520 PMT-1, 500V Pixel size: 50μm Detection of micro RNAs by primer extension method Micro RNAs in total RNAs dirived from mouse liver were detected by primer extension method. 5'-32P labeled oligo DNA (15 mer) complementary to each micro-RNA was used as a primer. Arrows indicate signals of cDNAs terminated at 5' end of micro RNAs. Sample: mouse total RNA Nuclides: 32P Exposing time: one night Excitation wavelength: 650nm Filter: B390 PMT-1 Data:  courtesy of Graduate School of Engineering, the University of Tokyo Tsutomu Suzuki, Ph.D., Tomomi Orito Multiple fluorescent dyes labeled protein by SDS-PAGE Sample:  Green: Cy™3 labeled BSA Blue: Cy™2 labeled Carbonic anhydrase Red: Cy™5 labeled Lysozyme PMT-1 Sample: Cy™2 labeled Carbonic anhydrase Density:  90ng / 45ng / 22.5ng / 11.3ng / 5.6ng / 2.8ng / 1.4ng / 700pg / Excitation wavelength: 473nm Filter: Y520 PMT-1, 500V Pixel size: 100μm PAGE of Cy™5 labeled Lysozyme Sample: Cy™5 labeled Lysozyme Density:  356ng / 178ng / 89ng / 44.5ng / 22.3ng / 11.1ng / 5.6ng / 2.8ng / Excitation wavelength: 635nm Filter: R670 PMT-1, 500V Pixel size: 100μm Western Blotting detected by Alexa Fluor®680 Sample: Mouse IgG Density:  10ug, 5ug, 2ug, 1ug, 500ng, 200ng, 100ng, 50ng, 20ng, 10ng, 5ng, 2ng Excitation wavelength: 670nm Filter: R710 PMT-2, 1000V Pixel size: 50μm Western Blotting detected by DY682 Sample: BSA Primary antibody: Monoclonal Anti-BSA antibody produced in Mouse Secondary antibody: DY-682-Goat anti-Mouse LgG Excitation wavelength: 670nm Filter: R710 PMT-2, 1000V Pixel size: 50μm CBB stained 2D gel electrophoresis of Saccharomyces cerevisiae 1st dimension:  Low pH isoelectric electrophoresis kit (Nacalai Tesque, Inc.) 2nd dimension:  17% low BIS SDS-PAGE Sample: Saccharomyces cerevisiae Excitation wavelength: 650nm Filter: O580 PMT-2, 500V Pixel size: 50nm Data:  courtesy of Yoshihiro Yamamoto, Industrial Technology Center, Kyoto Municipal Industrial Research Institute SYPRO® Orange stained 2D gel electrophoresis of Saccharomyces cerevisiae 1st dimension:  Low pH isoelectric electrophoresis kit (Nacalai Tesque, Inc.) 2nd dimension:  17% low BIS SDS-PAGE Sample: Saccharomyces cerevisiae Excitation wavelength: 473nm Filter: Y520 PMT-2, 500V Pixel size: 50μm Data:  courtesy of Yoshihiro Yamamoto, Industrial Technology Center, Kyoto Municipal Industrial Research Institute Whole body autoradiography to analyze metabolism of 14C labeled compound Nuclides: 14C IP: BAS-MS2040 Excitation wavelength: 650nm Filter: B390 PMT-2, 900V Pixel size: 50μm Data: courtesy of Institute of Whole Body Metabolism To FLA-7000