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An image taken 24 hours intravenous injection of DY676-labeled antibody*(Overlapped image: Near-infrared fluorescence image + digitizing image)
Near-infrared fluorescence image
Reagent: DY675
Light source: IR LED (710nm) epi-illuminator
Filter: R785
Exposure time: 60 sec.
*antibody: an antibody directed against a human cell surface marker was supplied by Perseus Proteomics Inc. (http://www.ppmx.com)
Digitizing image
Light source: White LED epi-illuminator
Filter: none
Exposure time: 0.01 sec. |
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Western Blotting with AlexaFluor®680
Sample: BSA
Reagent: AlexaFluor®680
Sensitivity mode: Standard
Light source / Filter: IR LED (710nm) epi-illuminator / IR785
Exposure time: 60 sec. |
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BSA labeled with AlexaFluor®750
Method: Western blotting
Light source: IR LED (710nm) epi-illuminator
Filter: IR785
Exposure time: upper 2 min. lower 10 min. |
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Mouse IgG reacted with anti-Mouse IgG-conjugate labeled with Qdot® 605
Reagent: Qdot® 605
Light source: UV LED(365nm) epi-illuminater
Filter: 605DF40
Exposure time: 60 sec. |
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λ DNA / Hind III stained with EtBr
Method: Agarose gel electrophoresis
Reagent: EtBr
Light source: UV LED (365nm) epi-illuminator
Filter: 605DF40
Exposure time: 1 min. |
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λ DNA / Hind III stained with EtBr
Method: Agarose gel electrophoresis
Reagent: EtBr
Light source: UV (312nm) trans illuminator
Filter: 605DF40
Exposure time: 1 sec. |
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DNA labeled with Cy-5
Method: Poly-acrylamide electrophoresis
Reagent: Cy-5
Light source: Red LED (630nm) epi -illuminator
Filter: R670
Exposure time: 30 sec. |
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DNA labeled with Cy-3
Method: Poly-acrylamide electrophoresis
Light source: Green LED (520nm) epi-illuminator
Filter: 575DF20
Exposure time: 30 sec. |
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λ / Hind III
Reagent: SYBR® Green
Light source: Blue LED (460nm) epi-illuminator
Filter: Y515-Di
Exposure time: 5sec. |
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Agarose gel electrophoresis of diluted series of λ DNA / Hind III
Reagent: SYBR® Green I
Light source: Blue LED (460) epi-illuminator
Filter: Y515-Di
Exposure time: 20 sec. |
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GFP expressed colony and GFP non-expressed colony
Light source: Blue LED (460nm) epi-illuminator
Filter: upper 510DF10, lower Y515-Di
*Image of GFP expressed colony with 510DF10 filter is more discernible than that with Y515-Di filter. |
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Chemiluminescent Western Blotting using ECL™
Sample: Dilution series of mouse IgG
Exposure time: 30 sec |
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Mouse IgG reacted with anti-Mouse IgG-peroxidase-conjugate
Method: Slot blotting
Membrane: PVDF
Reagent: ECL™
Exposure time: 5 min. |
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DIG-labeled control DNA hybridized with anti-DIG-AP, Fab fragment
Method: Dot blotting
Membrane: Nylon
Reagent: CDP-Star®
Exposure time: 2 min. |
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