FLA-7000

FLA-7000 is an advanced multipurpose bio-imaging analyzer which enables radioisotopic, fluorescent and digitized image capturing according to user's needs.
With maximum 4 kinds of onboard fluorescence excitation lasers, this system is able to cover a broader range of fluorescent dyes including near-infrared.


Fujifilm has discontinued sales of this product. An equivalent product is available through GE Healthcare.

Features

  • Multipurpose Imaging System suitable for RI, Fluorescent and digitized Imaging with High Resolutions

Features

Multipurpose Imaging System suitable for RI, Fluorescent and digitized Imaging with High Resolutions

Radioisotope

A Radioisotope-labeled sample image can be acquired with high throughput and high sensitivity using Fujifilm' s unique proprietary technology: Integrated Imaging Plate (IP) technology and high-speed detection technology (Light Collecting Guide).

The system ensures radioisotopic imaging with high sensitivity, which comes from Fujifilm's unique and reliable technologies. The IP consists of a radiosensitive layer of photostimulable phosphor crystals on a polyester support. The IP accumulates irradiated radiation energies on exposure for further scanning excitation process. The innovative and ergonomic design provides a broad range of advantages.

Fluorescence

A maximum of four lasers are optionally available for use with the following:
LD473nm, SHG532nm, LD635nm, LD650nm and LD670nm.
This enables the system to be upgraded to cover a broader range of fluorescent dyes including near-IR. LD650nm laser is already installed in the scanner.

  • Easy-to-access four filters (filters of Y520, O580, R670, R710, and a filter for IP) for upgrading flexibility.
  • Four optical filters are changeable by users, allows users to select appropriate fluorescent dyes for specific applications.

Digitizing

Able to take digitized images of Silver-stained gel or CBB-stained gel. (Gel Documentation)

Specifications

Overview

Measuring modes RI (IP detection),
Fluorescence (Detection of fluorescent dye by R,G,B NIR laser beams),
Digitizing (Gel documentation with Fluorescent plate)
Excitation 473nm, 532nm, 635nm, 650nm, 670nm
NOTE* The most suitable PMT is required depending on the combination of exciting lasers among two optional PMTs.
Nuclides for RI
Image detection		 14C, 32P, 33P, 35S, 3H, Neutron, etc.

Representative Fluorescent Dyes

473nm Laser Cy™2, FITC, SYPRO® Ruby, SYPRO® Orange, ECL plus™, silver stain, SYBR® Green I, SYBR® Green II, SYBR® safe, SYBR® Gold, FAM™, Alexa Fluor®488, ECF™
532nm Laser Cy™3, EtBr, ROX™, HEX™, TAMRA™, Alexa Fluor®546, CBB
635nm Laser Cy™5, Alexa Fluor®633
670nm Laser Alexa Fluor®680
Dynamic Range Five/Four orders of magnitudes changeable
Bit Depth:16bits(gray scale:65,536)
Scanning Size IP max; 20(W) X 40(D)cm (effective 18(W) X 36(D)cm)
Fluorescence max; 24(W) X 40(D)cm (effective 22(W) X 36(D)cm)
Pixel Size 25μm, 50μm, 100μm, 200μm
Filters Maximum four optional filters can be equipped;
Excitation of blue dye: Y520
Excitation of green dye: O580
Excitation of red dye: R670
Excitation of near-IR dye: R710
For IP: B390
Interface USB2.0

Operating System

  • Windows® and Mac™OS

Physical specifications

Dimensions 940(W) x 556 (D) x 360 (H) mm
Weight ca.62kg

Operating conditions

Supply voltage AC100-240± 10%
Power supply frequency 50/60Hz
Operating conditions Temperature; 15-30°C
Humidity: 30-70% RH (non-condensing)
Power consumption ca.0.3kVA

Optional Products

  • IP Eraser 3: Applicable up to 40 X46cm size

Accessories

  • PMT 1 (Bi-Alkali)
  • PMT 2 (Multi-Alkali)
  • 473nm Laser
  • 532nm Laser
  • 635nm Laser
  • 670nm Laser
  • Y520 Filter
  • O580 Filter
  • R670 Filter
  • R710 Filter
  • B390 Filter (for IP)
  • IP Stage
  • FLUOR stage (with Fluorescent Board)
  • Membrane weight

Image Data

Whole body autoradiography to analyze metabolism of 14C labeled compound
Nuclides
14C
IP
BAS-MS2040
Excitation wavelength
650nm
Filter
B390
PMT-1
Sensitivity
S10000
Pixel size
50μm
Data
courtesy of Institute of Whole Body Metabolism
Detection of modified bases in RNAs by primer extension method
Primer extension analysis of E. coli 23S rRNA which was treated with or without CMC(N-cyclohexyl-N ' -β-(4-methylmorpholinium)ethycarbodiimide p-tosylate) to detect pseudouridine (Ψ) (left panel). As a control (right panel), the same analysis was performed against E. coli strain in which rluD (RNA-pseudouridylase) was depleted. m3Ψ stands for 3-methyl pseudouridine.
Sample
ribosome RNA
Nuclides
32P
IP
BAS-MS2040
Exposing time
one night
Excitation wavelength
650nm
Filter
B390
PMT-1
Sensitivity
S10000
Pixel size
50μm
Data
courtesy of Graduate School of Engineering, the University of Tokyo
Tsutomu Suzuki, Ph.D., Naomi Hirabayashi
SYBR® Green stained agarose gel electrophoresis of DNA
Sample
λHindIII
Excitation wavelength
473nm
Filter
Y520
PMT-1, 500V
Pixel size
50μm
SYPRO® Ruby stained 2D gel electrophoresis of Saccharomyces cerevisiae
1st dimension
Low pH isoelectric electrophoresis kit (Nacalai Tesque, Inc.)
2nd dimension
17% low BIS SDS-PAGE
Sample
Saccharomyces cerevisiae
Excitation wavelength
473nm
Filter
O580
PMT-2, 500V
Pixel size
50μm
Data
courtesy of Yoshihiro Yamamoto, Industrial Technology Center, Kyoto Municipal Industrial Research Institute
PAGE of Cy™3 labeled primer
Sample
Cy™3 labeled primer
Density
500fmol / 200fmol / 100fmol / 50fmol / 20fmol / 10fmol / 5fmol / 2fmol / 1fmol
Excitation wavelength
532nm
Filter
O580
PMT-1, 800V
Pixel size
50μm
PAGE of Cy™5 labeled primer
Sample
Cy™5 labeled primer
Density
500fmol / 200fmol / 100fmol / 50fmol / 20fmol / 10fmol / 5fmol / 2fmol / 1fmol
Excitation wavelength
635nm
Filter
R670
PMT-1, 800V
Pixel size
50μm
Silver-stained protein by SDS-PAGE
Sample
BSA
Density
molecular-weight marker / 3μg / 1μg / 300ng / 100ng / 30ng / 10ng / 3ng / 1ng
Excitation wavelength
473nm
Filter
Y520
PMT-1, 500V
Pixel size
50μm
Detection of micro RNAs by primer extension method
Micro RNAs in total RNAs dirived from mouse liver were detected by primer extension method. 5'-32P labeled oligo DNA (15 mer) complementary to each micro-RNA was used as a primer.
Arrows indicate signals of cDNAs terminated at 5' end of micro RNAs.
Sample
mouse total RNA
Nuclides
32P
Exposing time
one night
Excitation wavelength
650nm
Filter
B390
PMT-1
Data
courtesy of Graduate School of Engineering, the University of Tokyo
Tsutomu Suzuki, Ph.D., Tomomi Orito
Multiple fluorescent dyes labeled protein by SDS-PAGE
Sample
  • Green: Cy™3 labeled BSA
  • Blue: Cy™2 labeled Carbonic anhydrase
  • Red: Cy™5 labeled Lysozyme
PMT-1
Sample : Cy™2 labeled Carbonic anhydrase
Density
90ng / 45ng / 22.5ng / 11.3ng / 5.6ng / 2.8ng / 1.4ng / 700pg /
Excitation wavelength
473nm
Filter
Y520
PMT-1, 500V
Pixel size
100μm
PAGE of Cy™5 labeled Lysozyme
Sample
Cy™5 labeled Lysozyme
Density
356ng / 178ng / 89ng / 44.5ng / 22.3ng / 11.1ng / 5.6ng / 2.8ng /
Excitation wavelength
635nm
Filter
R670
PMT-1, 500V
Pixel size
100μm
Western Blotting detected by Alexa Fluor®680
Sample
Mouse IgG
Density
10ug, 5ug, 2ug, 1ug, 500ng, 200ng, 100ng, 50ng, 20ng, 10ng, 5ng, 2ng
Excitation wavelength
670nm
Filter
R710
PMT-2, 1000V
Pixel size
50μm
Western Blotting detected by DY682
Sample
BSA
Primary antibody
Monoclonal Anti-BSA antibody produced in Mouse
Secondary antibody
DY-682-Goat anti-Mouse LgG
Excitation wavelength
670nm
Filter
R710
PMT-2, 1000V
Pixel size
50μm
CBB stained 2D gel electrophoresis of Saccharomyces cerevisiae
1st dimension
Low pH isoelectric electrophoresis kit (Nacalai Tesque, Inc.)
2nd dimension
17% low BIS SDS-PAGE
Sample
Saccharomyces cerevisiae
Excitation wavelength
650nm
Filter
O580
PMT-2, 500V
Pixel size
50μm
Data
courtesy of Yoshihiro Yamamoto, Industrial Technology Center, Kyoto Municipal Industrial Research Institute
SYPRO® Orange stained 2D gel electrophoresis of Saccharomyces cerevisiae
1st dimension
Low pH isoelectric electrophoresis kit (Nacalai Tesque, Inc.)
2nd dimension
17% low BIS SDS-PAGE
Sample
Saccharomyces cerevisiae
Excitation wavelength
473nm
Filter
Y520
PMT-2, 500V
Pixel size
50μm
Data
courtesy of Yoshihiro Yamamoto, Industrial Technology Center, Kyoto Municipal Industrial Research Institute
Whole body autoradiography to analyze metabolism of 14C labeled compound
Nuclides
14C
IP
BAS-MS2040
Excitation wavelength
650nm
Filter
B390
PMT-2, 900V
Pixel size
50μm
Data
courtesy of Institute of Whole Body Metabolism

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