FLA-8000

The Fujifilm FLA-8000 Fluorescent Image Analyzer features fluorescent and radioisotope image detection in a single laser imaging system. This powerful new research tool captures images of samples labeled with fluorescent dyes or radioisotopes at resolutions meeting the needs of the most demanding laboratories. Speed, image quality and flexibility make the FLA-8000 a remarkable imaging system with extraordinary capabilities.


Sales of this product have been discontinued.

Features

  • Capture high-resolution fluorescent and radiosotope images for versatile bioresearch methodologies.

Features

Capture high-resolution fluorescent and radiosotope images for versatile bioresearch methodologies.

Proven, patented technology.

Fujifilm accumulated more than 15 years of experience with Phosphor Imaging Plate (IP) technology, then applied that technology to uses in bioresearch with the innovative FLA-3000 imaging system. The FLA-8000 builds on this experience and takes it to the next level with unheard of resolutions for Radioisotope (RI) and fluorescent image detection.

Multiple lasers add imaging applications.

The FLA-8000 system includes two standard fixed lasers: a Second Harmonic Generation (SHG) green laser (532nm) and a Laser Diode (LD) red laser (635nm). A SHG laser (blue-473nm) is available as an option for additional applications. Fujifilm's patented laser technology provides superior imaging speed, throughput, sensitivity and resolution. Typical fluorescence applications include: SYPRO® Orange, SYPRO® Ruby, SYBR® Green I, FITC, Cy3™, Rhodamine, SYPRO® Red and Cy5™. In addition, the system is the only multifunctional scanner that not only efficiently detects and discriminates between Cy3™ and Cy5™, but also between Cy2™ and Cy5™ labeled samples.

[Photo] Multiple lasers add imaging applications.

Molecular Probes FluoCells F-14780 Mito Tracker Red CMXRos F532nm F675DF20 BODIPY FL phallacidin F473nm F530DF20 (FLA-8000 setting: 5µm pixel size)

  • * No license is granted for the use in combination with fluorescent microarray glass slide.

New imaging plate (IP) adds flexibility and economy.

In addition to fluorescent-dye stained gel images excited by either the fixed 532nm SHG or 635nm LD laser, the FLA-8000 captures radioisotopic images with the newly developed BAS-SR0813 Imaging Plate. Fujifilm Imaging Plates have a flexible polyester base coated with highly dispersed barium fluoro-halide phosphor crystals. When exposed to a radioactively labeled sample, the energy is transferred to the plate. The plate is then scanned by one of the FLA-8000 lasers. Through a process known as photo-stimulated luminescence, blue light is emitted and collected to produce a high-resolution digital image. The IPs are erasable for reuse.

[Photo] New imaging plate (IP) adds flexibility and economy.

Data courtesy of Dr. Makoto Mochii,Department of Life Science, Faculty of Science,Himeji Institute of Technology, Japan.33P labeled probe was hybridized with 9600 dots cDNA on 8.5 x 12.7cm membrane. (FLA-8000 setting: 20µm pixel size)

Improved resolution capabilities extend laboratory flexibility.

Image resolution (pixel size) on the FLA-8000 has been significantly improved over earlier imaging systems. User-selectable pixel size can be set as low as 5 microns for fluorescent images or 10 microns for radioisotopic images. Gel images are non-selectable at 100 microns. With extremely fine pixel sizes and a wide dynamic range of five orders of magnitude, the detail offered by the FLA-8000 affords a broad range of applications from gene array analysis to drug metabolism. The system is fast, even at the highest resolutions, and the patented lasers emit coherent light that results in the best image quality, even with thick gels.

Versatile image capturing.

Three types of sample carriers allow a wide range of image capturing capabilities. The slide carrier allows five 74mm x 23mm glass slides to be concurrently scanned at 5, 10 or 20 micron pixel size. The 85mm x 127mm IP carrier provides a fast and accurate means for quantitative analysis of all alpha, beta and gamma emitting isotopes at pixel sizes of 10, 20, 50 or 100 microns. The gel carrier provides simultaneous scanning of two 51mm x 59mm gel sheets at a fixed pixel of 100 microns.

Specifications

Image Reading Software

  • Windows®

Lasers

Two fixed lasers for fluorescent dye excitation and reading radioisotopic image 532nm SHG
635nm LD
One optional laser for fluorescent dye excitation 473nm SHG

Nuclides for RI Image Detection

  • 14C, 32P, 33P, 35S, 125I

Representative Fluorescent Dyes

473nm SHG Laser SYPRO® Orange,
SYPRO® Ruby,
SYBR® Green I,
FITC
532nm SHG Laser Cy3™,
Rhodamine,
SYPRO® Red
635nm Laser Cy5™

Dynamic Range

  • Five orders of magnitude

Bit Depth

  • 16 bits (gray scale: 65, 536)

Recommended Use/Speciman

RI Image Radioisotope-labeled macroarray membrane
In situ hybridization
Radioisotope-labeled electrophoresis gel
Radioisotope-labeled rat brain autoradiography
Fluorescent Image Fluorescent dye stained gel(mini-gel)
Fluorescent dye labeled electrophoresis
FISH on glass slide
GFP image for protein analysis
  • * No license is granted for the use in combination with fluorescent microarray glass slide.

Sample Size and Scanning Size

  SAMPLE SIZE
(mm)		 SCANNING SIZE
(mm)		 PIXEL SIZE
(micron)
Slide Carrier 5 glass slides 5x(74x23) 5/10/20
IP Carrier 85x127 IP 85x127 10/20/50/100
Gel Carrier 2 gel sheets(51x 59) 2x(50x58) 100 only

Filters

Three fixed filters for modularity 530nm
570nm
675nm
One optional filter chosen by user 25mmø t2-t12mm
  • * In case of IP detection, this position is occupied by a B390 filter for photo-stimulated luminescence.

Dimensions

  • 850(W) x 730(D) x 480(H)mm

Weight

  • ca. 110kg

Modularity

The most suitable FLA-8000 can be upgraded depending on your purpose by selecting any optional component.

Optional Components

  • 473nm SHG laser
  • Sample carriers
  • (IP Carrier, Slide Carrier, Gel Carrier)
  • IP cassette
  • Filter (B390 for IP reading)
  • BAS-SR0813 IP
  • IP Eraser 3

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