FLA-9000

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Additionally, the new 785nm laser affords all the advantages of near-infrared imaging. And now you can experience the actual operation, which complies fully with 21 CFR Part11.

STARION is the ultimate multi-purpose image scanner. Covering Fluorescent, Radioisotopic, Chemiluminescent and Digitized images with enhanced modality to meet your life science needs.


Fujifilm has discontinued sales of this product. An equivalent product is available through GE Healthcare.

Features

  • THE STARION IS A NEXTGENERATION,MULTI-PURPOSE IMAGE SCANNER.

Features

THE STARION IS A NEXTGENERATION,MULTI-PURPOSE IMAGE SCANNER.

All in one system

Radioisotopic, Fluorescent, Chemiluminescent and Digitized images can be captured in High Resolution using the minimum 10µm pixel size. The maximum scanning area of 40 x 46cm, and easy filter exchange, support a wide range of reagents and applications.
The system supports USB2.0 and the all-in-one set up saves space in your laboratory.

Laser upgradeable

The system supports 473nm, 532nm, 635nm, 685nm and 785nm lasers and is flexible enough to allow you to install four different lasers at once. The improved 473nm Blue laser diode light source enables you to capture vivid images of Cy™2-labeled gels and blots.
Additionally, the new 685nm and 785nm lasers afford all the advantages of near-infrared imaging. Fujifilm’s STARION supports a wide array of optional accessories to meet your application needs.

STARION Setting Example

Please consult with your local distributor on other available settings and combinations.

Fluorescence reagents corresponding to excitation wavelength

Advanced imaging

The STARION is a high quality image scanner supporting a wide variety of applications from traditional radioisotopic imaging using imaging plates to highly sensitive fluorescent applications like 2D gel analysis. The system supports multispectral fluorescent applications such as Western blotting with Qdot® or Alexa Fluor® dyes, delivering all the expected benefits of imaging in the near-IR. Additionally, the system allows for parallel detection using two excitation wavelengths simplifying the workflow for multispectral applications.

Specifications

FLA-9000 Reader

Detection mode Fluorescence, RI (Radioisotope),
Digitizing,Chemiluminescence
Excitation wavelength 473nm (Blue LD laser), 532nm (Green SHG laser),
635nm (Red LD laser), 685nm (Red LD laser),
785nm (Near-IR LD laser)
Nuclides 3H, 14C, 32P, 33P, 35S, 125I, Neutron, etc.
Dynamic Range Five orders of magnitudes
Bit depth 8bit / 16bit
Scanning size 40 x 46cm
Pixel size User choice of 10, 25, 50, 100, 200 micron.
Filters B390 (IP)
LPB (510LP), LPG (575LP), LPR (665LP)
BPB (530DF20), BPG (570DF20), BPFR700 (R715), BPFR800 (R810)
DBR (530DF20 / 665LP), DGR (570DF20 / 665LP)
Dimensions 900 (W) x 800 (D) x 400 (H) mm
Weight ca. 97 kg

FLUOR Stage (with optional gel stopper)

A glass plate of 40 x 46cm can be used for fluorescence detection, digitizing function and chemiluminescence detection.

IP Stage

A magnetic stage holds the IP with a soft ferrite backing layer of any size up to 40 x 46cm.

MULTI Stage (with optional microtiter plate holder, which can hold up to six microtiter plates)

Large size polyacrylamide gel plate can be measured with the glass.

IP Eraser 3

  • applicable up to 40 x 46cm size

PMT1

  • For Blue, Green, Red laser

PMT2

  • For Red, IR laser

Imaging Plate

  • BAS-MS 2025/ 2040/ 2325/ 2340/ 3543
  • BAS-SR 2025/ 2040
  • BAS-TR 2025/ 2040
  • BAS-ND 2025/ 2040

BAS cassette

Operating System

Windows® XP Professional SP2, Vista Business
Macintosh® Mac® OS X 10.4.10, Intel Mac only,
(unavailable with Power Mac)

Image capture software

  • ImageReader FLA-9000

Analysis software

  • Science Lab

Operating conditions

Line frequency 50 / 60Hz
Temperature 15-30°C
Humidity 20-70% (no condensation)
Supply voltage AC100-240V ±10%
Power consumption Approx. 0.3kVA

Image Data

1D gel of Whole blood proteins labeled with Cy™2
FLA-9000
Ex: 473nm, Filter: LPB
PMT
500V
Pixel size
25μm
2D gel of human brain lysates labeled with Cy™2
FLA-9000
Ex: 473nm, Filter: LPB
PMT
500V
Pixel size
25μm
Fluorescence detection of Cy™2,Cy™3 and Cy™5 superimposed by Multi Gauge software.
Sample
1D PAGE of Cy™2 conjugated Carbonic anhydrase, Cy™3 conjugated BSA and Cy™5 conjugated lysozyme
FLA-9000
Ex: 473nm, Filter: LPB
Ex: 532nm, Filter: LPG
Ex: 635nm, Filter: LPR
PMT
400V
Pixel size
200μm
The images were obtained sequentially bycyclic mode and composed by MultiGauge software.
Fluorescence detection of SYPRO® Ruby-stained 2D gel
Sample
Sake yeast
FLA-9000
Ex 473nm, Filter: LPB
PMT
500V
Pixel size
50μm
Fluorescence detection of Pro-Q®Diamond
Sample
α-Casein
FLA-9000
Ex: 532nm, Filter: LPG
PMT
400V
Pixel size
100μm
Fluorescence detection of AlexaFluor® 680 Western blotting
Sample
BSA
FLA-9000
Ex: 685nm, Filter:BPFR700
PMT
1000V
Pixel size
100μm
Fluorescence detection of AlexaFluor®750 Western blotting
Sample
BSA
FLA-9000
Ex: 785nm, Filter: BPFR800
PMT
1000V
Pixel size
100μm
Fluorescence detection of IRDye® 700 Western blotting
Sample
BSA
FLA-9000
Ex: 685nm, Filter: BPFR700
PMT
1000V
Pixel size
100μm
Fluorescence detection of IRDye® 800 Western blotting
Sample
BSA
FLA-9000
Ex: 785nm, Filter: BPFR800
PMT
1000V
Pixel size
100μm
Fluorescence detection of DY-682 Western blotting
Sample
BSA
FLA-9000
Ex: 685nm, Filter: BPFR700
PMT
1000V
Pixel size
100μm
Fluorescence detection of Cy™2 Titerplate
Sample
Cy™2 labeled Primer
FLA-9000
Ex: 473nm, Filter: LPB
PMT
1000V
Pixel size
100μm
Digitization of CBB-stained 2D gel
Sample
Sake yeast
FLA-9000
Ex: 532nm, Filter: LPG
PMT
250V
Pixel size
100μm
Whole body autoradiography of a rat
Sample
14C-labeled drug-injected rat
FLA-9000
IP-S mode
Pixel size
50μm
IP
BAS-MS type
Rat brain autoradiography by BAS-TR plate
Sample
3H-labeled receptor assay
FLA-9000
IP-S mode
Pixel size
25μm
IP
BAS-TR type