LAS-4000
This model succeeded LAS-3000 which was equipped with Fujifilm's proprietary high sensitive and high resolution CCD technology and further evolved to the system with various capable applications with wider line-ups of optional product. In addition to the existed epi-blue, green and red illuminators, epi-UV and epi-IR illuminators are lined up. Since near infrared rays from epi-IR illuminator well penetrate live tissue, in vivo imaging of small animal, such as mouse, with high sensitivity and high resolution is available.
Fujifilm has discontinued sales of this product. An equivalent product is available through GE Healthcare.
Features
- Improved sensitivity and resolution
- Improved versatility
- Improved user interface
- Improved Image Reader software
Features
This system is especially suitable for Western blotting analysis. High sensitive chemiluminescent detection was designed to compete with the X-ray films. A wide variety of excitation light sources enables this system to capture various fluorescent images, including in vivo imaging or fluorescent Western blotting, also.
- Improved sensitivity and resolution
- Improved versatility
- Improved user interface
- Improved Image Reader software
Improved sensitivity and resolution
New Super CCD Area Type Imaging Chip
This system is especially suitable for Western blotting analysis.High sensitive chemiluminescent detection was designed to compete with the X-ray films. Image acquire and digitize can capture both chemiluminescence and digitized marker image by one click.
New Super CCD Area Type Imaging Chip - The higher sensitivity of Fujifilm's new CCD provides improved faint-light image capture with outstanding image clarity. The new chip contains 3.2M pixels of which 3.15M contribute to actual imaging. The increased pixel size results from the use of octagonal-shaped photodiodes, which capture a great deal of light. The superior image sensing capability of the new chip results in ultra-high resolution image files of up to 6.3M pixels (3,072 x 2,048 pixels).
Super CCD
By rotating pixels 45 degrees to form an interwoven layout, the Super CCD's pixel pitch in the horizontal and vertical directions is narrower than in the diagonal direction, achieving higher horizontal and vertical resolution.
Conventional CCD
With conventional interline CCDs, pixel pitch in the diagonal direction is narrower than in the horizontal and vertical directions, resulting in higher diagonal resolution.
New Binning Mode
The LAS-4000 features a new binning mode, which allows users to effectively increase the pixel size and, therefore, the imaging sensitivity of the system.
Additionally, the process significantly enhances the signal-to-noise ratio. The system allows four binning settings: 1x2 (standard), 2x4 (high), 4x8 (super) and 8x16 (ultra). Images obtained in the binning mode are further enhanced by software-based pixel supplement to compensate for any loss in resolution.
The binning mode of the LAS-4000 allows researchers to select from four binning settings to enhance both imaging sensitivity and image resolution. With the Image Reader Pro software, High resolution, High binning, Super binning and Ultra binning images can be obtained in addition to the standard High Super and Ultra images.
Exposure Options
The extremely fast, electronically cooled, high-resolution camera component allows long exposure times in any laboratory setting. Exposures can be set from 1/100th of a second to 30 hours to meet a wide range of imaging conditions. The 30-hours exposure function allows over-night exposures on very dark images.
Improved versatility
New Filter Options
A 5-position filter turret is mounted in front of the lens, which allows convenient, electrically controlled filter selection in less than ten seconds. With Image Reader software, the user has control over three standard filters (605DF40 for EtBr, Y515 for SYBR® Green and 510DF10 for GFP), a fourth filter position for chemiluminescence and a fifth user-specified filter for fluorescence. With Image Reader software, the user has full control over four filter types and positions. Other than the above, 575DF20 filter was prepared for green LED, L41 filter was prepared for UV LED, and IR785 filter was prepared for IR LED.
Additional Light Sources
The number of light sources on the LAS-4000 system has been increased to eight, including UV transilluminator, white transilluminator, epi-blue illuminator, epi-green illuminator, epi-red illuminator, epi-UV illuminator, epi-IR illuminator, epi-white illuminator. With ImageReader software, the user has control over eight light sources (UV transilluminator, white transilluminator, epi-blue illuminator, epi-green illuminator, epi-red illuminator, epi-UV illuminator, epi-IR illuminator and epi-white illuminator).
Sampling Convenience
The LAS-4000 accommodates samples up to 25 x 25cm in size with an optional wide view lens. Various types of trays are prepared such as an EPI Tray for chemiluminescence and epi-illuminator, a DIA Tray for UV trans-illuminator , a white-DIA Tray for white trans-illuminator such as CBB stained gel, and a non-parallax (NP) Tray for up to two titer plates. The NP tray includes a Fresnel lens located between the titer plate and the lens, which eliminates parallax in each of the 96 titer plate wells. NP Tray is designed for the detection of chemiluminescence.
Improved user interface
Simplified User Interface
The system functions of the LAS-4000 are controlled remotely through either a Mac™ or Windows® software application via a standard USB2.0 interface.
Image capture and analysis software is provided. The newly simplified user interface allows researchers to spend more time in research and less time in system integration and maintenance.
Light-tight operation
The light-tight dark box affords highly sensitive imaging from a variety of light sources in an ordinary lab. After placing the sample tray in the dark box and closing the light-tight door, all imaging functions are controlled remotely from the PC. With the keyboard and monitor, the researcher conveniently adjusts focus, selects the appropriate filter and sets the exposure to capture the image.
External cable / tube insertion port
The analyzer has a port for inserting temperature adjustment / anesthesia cables and tubes.
Improved Image Reader software
The self-explanatory and user-friendly software allows specification of all image capture parameters, including sensitivity, resolution and image methods. The software automatically carries out all image correction functions. In increment mode, the signal increase is shown in real time, by capturing and automatically displaying up to 16 images. With this software users can create their own settings and methods. A photograph of the sample can be made and overlaid with the signal image.
Specifications
Image Capturing Unit (Requires additional Analyzing Unit)
Camera head
| CCD chip | Fujifilm Super CCD Area Type chip (15.6 x 23.4mm) |
|---|---|
| Number of pixels | 3.2 million pixels |
| Pixel size | Approx. 11 µm |
| Cooling | Two-stage thermoelectric module with air circulation |
| Cooling temperature | Down to -30°C (When room temperature is below 28°C.) |
| Dynamic range | Four orders of magnitude |
| Focusing and diaphragm | Automatic, remote operation |
| Gradation | 16 bits |
| Exposure mode | Automatic / manual (normal / incremental / repetitive / program / in vivo) |
| Exposure time | Automatic / manual (1 / 100 seconds to 30 hours) |
| Pixel correction | Dark-frame correction, flat-frame correction, distortion correction, etc. |
| Image quality correction | Binning and smoothing |
| Image size | Up to 12 MB (formats : FUJI and TIFF) |
| Read pixel size | Down to 35 µm |
| Maximum sample size | 21 x 14 cm (25 x 25 cm when using a wide-view lens) |
| Interface | USB2.0 |
Intelligent Dark Box Set
- Includes; IDX box, Epi-tray, USB Cable
Lens
| High-sensitivity lens | FUJINON Lens VRF43LMD 3 |
|---|
- * SIGMA Wide-view Lens is also available.
Operating conditions
| Line frequency | 50 - 60 Hz |
|---|---|
| Temperature | 15 - 28℃ |
| Humidity | 30 - 70% (no condensation) |
| Supply Voltage | 100 - 240V |
| Power Consumption | Approx. 0.3 kVA |
Dimensions
| Camera head and dark box | 510 (W) x 900 (H) x 480 (D) mm |
|---|
Optional Products*
| UV 2020 Transilluminator set | Includes; UV Transilluminator(312nm), Gel sheet, UV Dia-tray, 605DF40 filter |
|---|---|
| White Transilluminator(470~740nm) set | Includes; White Trans-illuminator, White Dia-tray |
| Epi-Blue Illuminator set | Includes; Epi-Blue(460nm) / White Illuminator, Y515 filter |
| Epi-Green Illuminator set | Includes; Epi-Green(520nm) / White Illuminator, 575DF20 filter |
| Epi-Red Illuminator set | Includes; Epi-Red(630nm) / White Illuminator, R670 filter |
| Epi-IR Illuminator set | Includes; Epi-IR(710nm) / White Illuminator, IR785 filter |
| Epi-UV Illuminator set | Includes; Epi-UV(365nm) / White Illuminator, L41 filter |
| UV Dia-tray set | Includes; UV Dia-tray, Gel sheet |
| Non-parallax tray | |
| Optical Filter: Y515 |
|
| Filter changer |
- * Each light source set includes filter(s) for the corresponding wavelength range(s). The three optional filters can be purchased individually.
Analyzing Unit (separate product)
Computer
| OS |
|
|---|
Analysis software
- ScienceLab (MultiGauge)
Applicable reagents
| Luminescence | ECL™, ECL Plus™, ECL Advance™, Lumi-Light Plus, SuperSignal®, CDP-Star®, CSPD®, Immobilon, Bright-Star™ |
|---|---|
| Fluorescence / IR | DY676, DY781, Alexa Flour®680, Alexa Flour®700 |
| Fluorescence / red | Alexa Flour®633, Alexa Flour®635, Alexa Flour®647, Cy™5, BODIPY®650/665, DiD, TOTO®-3, DDAO phosphate |
| Fluorescence / green | SYPRO®Red, Cy™3, TAMRA™, ROX™, HEX™, Alexa Fluor®532, Alexa Fluor®546, Deep Purple, Pro-Q® Diamond, Rhodamine Red™, BODIPY®576/589, NED™, R-phycoerythrin, RFP, HNPP, Tetramethylrhodamine |
| Fluorescence / blue | SYBR® Green I, SYBR® Green II, SYBR® Gold, SYPRO® Ruby, SYPRO® Orange, SYPRO® Tangerine, FITC, FAM™, EGFP, ECFP, AttoPhos™ |
| Fluorescence/epi-UV | Ethidium Bromide, SYPRO® Rose, Qdot® |
| Fluorescence/trans-UV | Ethidium Bromide |
| Digitizing/white epi-illumination/white transillumination | Silver staining, CBB, NBT/BCIP, X-ray film, etc. |
- * The reagents listed above are representative reagents with high performance results. Please contact us for other reagents.
Remarks
Gel documentation is available by digitizing
An optimum system for meeting your needs can be constructed by freely combining a wide array of options with the basic model*.
- * Basic model: Camera Head + Intelligent Dark Box set + Lens
LAS-4000 (with any Epi-illuminator) is categorized as the class 1 laser (LED) (IEC60825-1+A2:2001).
- Notice
- With regard to patents owned by third parties related to, among other things, sample preparation, we recommend that you consult with a lawyer or patent attorney about obtaining a license from the third parties.
Image Data
- An image taken 24 hours intravenous injection of DY676-labeled antibody*(Overlapped image: Near-infrared fluorescence image + digitizing image)
-
Near-infrared fluorescence image
- Reagent
- DY675
- Light source
- IR LED (710nm) epi-illuminator
- Filter
- R785
- Exposure time
- 60 sec.
Digitizing image
- Light source
- White LED epi-illuminator
- Filter
- none Exposure time: 0.01 sec.
- * antibody: an antibody directed against a human cell surface marker was supplied by Perseus Proteomics Inc. (http://www.ppmx.com)
- Western Blotting with AlexaFluor®680
-
- Sample
- BSA
- Reagent
- AlexaFluor®680
- Sensitivity mode
- Standard
- Light source / Filter
- IR LED (710nm) epi-illuminator / IR785
- Light source / Filter
- IR LED (710nm) epi-illuminator / IR785
- BSA labeled with AlexaFluor®750
-
- Method
- Western blotting
- Light source
- IR LED (710nm) epi-illuminator
- Filter
- IR785
- Exposure time
- upper 2 min. lower 10 min.
- Mouse IgG reacted with anti-Mouse IgG-conjugate labeled with Qdot® 605
-
- Reagent
- Qdot® 605
- Light source
- UV LED(365nm) epi-illuminater
- Filter
- 605DF40
- Exposure time
- 60 sec.
- λDNA / Hind III stained with EtBr
-
- Method
- Agarose gel electrophoresis
- Reagent
- EtBr
- Light source
- UV LED (365nm) epi-illuminator
- Filter
- 605DF40
- Exposure time
- 1 min.
- λ DNA / Hind III stained with EtBr
-
- Method
- Qdot® 605
- Reagent
- EtBr
- Light source
- UV (312nm) trans illuminator
- Filter
- 605DF40
- Exposure time
- 1 sec.
- DNA labeled with Cy-5
-
- Method
- Poly-acrylamide electrophoresis
- Reagent
- Cy-5
- Light source
- Red LED (630nm) epi -illuminator
- Filter
- R670
- Exposure time
- 30 sec.
- λ / Hind III
-
- Reagent
- SYBR® Green
- Light source
- Blue LED (460nm) epi-illuminator
- Filter
- Y515-Di
- Exposure time
- 5sec.
- Agarose gel electrophoresis of diluted series of λDNA / Hind III
-
- Reagent
- SYBR® Green I
- Light source
- Blue LED (460) epi-illuminator
- Filter
- Y515-Di
- Exposure time
- 20 sec.
- GFP expressed colony and GFP non-expressed colony
-
- Light source
- Blue LED (460nm) epi-illuminator
- Filter
- upper 510DF10, lower Y515-Di
- * Image of GFP expressed colony with 510DF10 filter is more discernible than that with Y515-Di filter.
- Chemiluminescent Western Blotting using ECL™
-
- Sample
- Dilution series of mouse IgG
- Exposure time
- 30 sec
- Mouse IgG reacted with anti-Mouse IgG-peroxidase-conjugate
-
- Method
- Slot blotting
- Membrane
- PVDF
- Reagent
- ECL™
- Exposure time
- 5 min.
- DIG-labeled control DNA hybridized with anti-DIG-AP, Fab fragment
-
- Method
- Dot blotting
- Membrane
- Nylon
- Reagent
- CDP-Star®
- Exposure time
- 2 min.
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