LAS-4000mini

This model succeeded LAS-3000mini which was equipped with Fujifilm's proprietary high sensitive and high resolution CCD technology and further evolved to the system with various capable applications with wider line-ups of optional product, such as epi-blue illuminator, epi-white illuminator or UV trans-illuminator. In addition to all-in-one compact body, quick focusing by control from a computer and quick response by USB 2.0 interface are available.


Fujifilm has discontinued sales of this product. An equivalent product is available through GE Healthcare.

Features

  • Super CCD chip affords up to 6.3 million pixels
  • Equipped with a newly designed large-aperture F0.85 lens
  • High-grade performance in a compact body
  • Image capture software "LAS-4000mini image Reader"

Features

Super CCD chip affords up to 6.3 million pixels

By rotating pixels 45 degrees to form an interwoven layout, the Super CCD's pixel pitch in the horizontal and vertical directions is narrower than in the diagonal direction, achieving higher horizontal and vertical resolution.

[Photo] Super CCD chip

Super CCD chip

[Figure] Octagonal pixel-interwoven layout

Octagonal pixel-interwoven layout

Equipped with a newly designed large-aperture F0.85 lens

The analyzer incorporates a FUJINON, a strikingly bright lens with an F-number of 0.85. This lens has been especially designed to make full use of the advantages of Fujifilm's proprietary Super CCD chip, and is excellent for capturing images from distances as short as several tens of centimeters.
In its design, optical expertise developed through professional applications such as broadcasting TV camera lens is fully exploited.

[Photo] FUJINON LENS VRF43LMD 3 F0.85

FUJINON LENS VRF43LMD 3 F0.85

High-grade performance in a compact body

The same grade of camera and lens as in LAS-4000, the highest-grade model of the LAS series, are used. A compact body is made possible while still retaining the high resolution and high performance of LAS-4000. Free from the necessity for dark rooms and chemicals, the analyzer can be placed on the top or side of an ordinary desk, and requires no special expertise for operation.

Image capture software "LAS-4000mini image Reader"

The self-explanatory and user-friendly software allows specification of all image capture parameters, including sensitivity, resolution and image methods. The software automatically carries out all image correction functions. In increment mode, the signal increase is shown in real time, by capturing and automatically displaying up to 16 images. With this software users can create their own settings and methods. A photograph of the sample can be made and overlaid with the signal image.

[Capture] Screen of Method/Tray position

Method/Tray position Screen

[Capture] Screen of Exposure Increment

Exposure Increment Screen

Optional Products

UV 2020 transilluminator set

Includes; UV 2020 transilluminator, 605DF40 optical filer for EtBr detection, UV-Dia tray, Gel sheet, etc.

UV-Bay set

Includes; Housing box for UV transilluminator, Filter frame, Filter housing, Bottom shield

EPI Blue Illuminator

Includes; EPI Blue / White Illuminator, Y515 optical filter, etc.

  • * The product with EPI Blue illuminator is categorized as the class 1 laser (LED) (IEC60825-1+A2:2001)

Optical Filter

  • 510DF10 for GFP detection
  • 605DF40 for EtBr detection
  • Y515

Non-parallax (NP) tray

A non-parallax (NP) tray is used to eliminate the parallax effect when imaging chemiluminescence in the 96-well plate. Up to two plates can be placed.

[Photo] NP Tray

Specifications

Image Capturing Unit (Requires additional Analyzing Unit)

Camera head

CCD chip Fujifilm Super CCD Area Type chip
Number of pixels 3.2 million pixels
Pixel size Approx. 11 µm
Cooling Two-stage thermoelectric module with air circulation
Cooling temperature Down to -30°C (When room temperature is below 28°C.)
Dynamic range Four orders of magnitude
Focusing and diaphragm Automatic, remote operation
Gradation 16 bits
Exposure mode Automatic / manual (normal / incremental / repetitive)
Exposure time Automatic / manual (1 / 100 seconds to 30 hours)
Pixel correction Dark-frame correction, flat-frame correction, distortion correction, etc.
Image quality correction Binning and smoothing
Image size Up to 12 MB (formats : FUJI and TIFF)
Read pixel size Down to 30 µm
Maximum sample size 18 x 12 cm
Interface USB2.0

Intelligent Dark Box LAS-4000mini

  • Includes; Epi-tray, USB Cable

Lens

High-sensitivity lens FUJINON VRF43LMD3 F0.85
  1. * Nikon F1.4 lens is also available.

Operating conditions

Line frequency 50 / 60 Hz
Temperature 15 - 28℃
Humidity 30 - 70% (no condensation)
Supply Voltage 100 - 240V
Power Consumption Approx. 0.3 kVA

Dimensions

Camera head and dark box 440 (W) x 670 (H) x 370 (D) mm 440 (W) x 750 (H) x 380 (D) mm / With UV transilluminator

Optional Products

UV 2020 Transilluminator set Includes; UV 2020 transilluminator, 605DF40 optical filter for EtBr detection, UV-Dia tray, Gel sheet
UV-Bay set Includes; Housing box for UV transilluminator, Filter frame, Filter housing, Bottom shield
Epi-Blue Illuminator set Includes; EPI Blue / White Illuminator, Y515 optical filter
Non-parallax tray
Optical Filter
  • 510DF10 for GFP detection
  • 605DF40 for EtBr detection
  • Y515
  • * The product with EPI Blue illuminator is categorized as the class 1 laser (LED) (IEC60825-1+A2:2001)

Analyzing Unit (separate product)

Computer

OS
  • Mac® OS X 10.4.4 or later
  • Windows® XP Pro SP2

Analysis software

  • ScienceLab (MultiGauge)

Applicable reagents

Chemiluminescence / Bioluminescence ECL™, ECL Plus™, ECL Advance™, Lumi-Light Plus, SuperSignal®, CDP-Star®, CSPD®, Renaissance, Bright-Star™, Luciferase
Fluorescence / Blue epi-illumination* SYBR® Green I, SYBR® Green II, SYBR® Gold, SYPRO® Ruby, SYPRO® Orange, SYPRO® Tangerine, FITC, FAM™, AttoPhos™
Fluorescence / trans-UV** Ethidium Bromide
Digitizing / White epi-illumination*** NBT / BCIP, Silver Staining, CBB
  • * excited by the Blue LED epi-illuminator
  • ** excited by the UV transilluminator
  • *** captured by the White LED epi-illuminator

Remarks

Gel documentation is available by digitizing

  • * The product with EPI Blue illuminator is categorized as the class 1 laser (LED) (IEC60825-1+A2:2001)
Notice
With regard to patents owned by third parties related to, among other things, sample preparation, we recommend that you consult with a lawyer or patent attorney about obtaining a license from the third parties.

Image Data

λ-DNA/Hind III stained with SYBR® Green I
Method
Agarose gel electrophoresis
Light sourcet
Blue LED epi-illuminator (460nm)
Filter
Y515-Di
Exposure time
1/2sec
BSA
Method
Polyacrylamide electrophoresis with SYPRO® Ruby
Light source
Blue LED epi-illuminator (460nm)
Filter
Y515-Di
Exposure time
3 sec.
λ-DNA/Hind III stained with EtBr
Method
Agarose gel electrophoresis
Light source
Trans-UV-illuminator (312nm)
Filter
605DF40
Exposure time
1/8sec
BSA
Reagent
ECL™
Sensitivity mode
Standard
Exposure time
3min.
Mouse IgG detected by Western blotting
Substrate
ECL™ Advance
Exposure time
4 sec.
Calcium-binding protein expressed in the rat small intestine, detected by Western blotting
Substrate
CSPD®
Exposure Time
10min
  • * Data courtesy of Laboratory of Molecular Nutrition Laboratory, Kagawa Nutrition University
Human IgG on a titer plate, detected by ELISA
Substrate
ECL™
Note
A non-parallax tray (optional product) was used.
Atlas™ Plastic Mouse 5K Microarray SpotLight™ Chemiluminescent Hybridization & Detection kit
Exposure time
10 min

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